Finnish Museum of Natural History
DNA laboratory at the Finnish Museum of Natural History
Facilities and equipment
The DNA laboratory facility at the Finnish Museum of Natural History (P. Rautatiekatu 13, 3rd floor) is equipped with necessary apparatuses for standard DNA extractions, PCR work and agarose gel electrophoresis and visualization. The laboratory offers most of the facilities required for basic molecular work, such as pipettes, centrifuges, fridges and freezers, biophotometer and hotblocks. Two deep freezers (situated in the freezer room in basement K2) are available for longtime storage of tissue and/or DNA samples. In addition, equipment for Cellulose-Acetate multilocus-allozyme gel electrophoresis will be acquired during 2009.
The main lab or 'pre-PCR' section of the laboratory is partitioned into ca 10 bench places. The places are allocated to different FMNH related research groups based on their current space requirements. During 2009 members of six FMNH research groups utilize the lab for molecular work. DNA extraction and PCR reaction setup work takes place in the main lab. The 'post-PCR' room of the laboratory is equipped with two fume hoods, ice machine, incubators, distilled and double-distilled water system and lab dishwasher.
The post-PCR room houses two Eppendorf Mastercycler S 96-well PCR machines, one 96-well and one 2x48 well MJ Research PTC PCR machines, and additionally a Bio-Rad Mini Cycler 48 well PCR machine. For agarose gel running the lab offers several running apparatuses of different capacities. Generally all steps involving work with PCR products are performed in this area.
The final sequencing or genotyping of the samples generated in the laboratory is by agreement with Department of Biological and Environmental Science performed in Viikki MES lab using the MegaBACE 1000 96-capillary system. The Megabace is used for both sequencing and genotyping (microsatellites, SNPs and SSCP) analyses. Alternatively sequencing samples are processed by commercial companies abroad. Sequence editing programs are available on Mac and PC computers.
For more information, please contact Gunilla Stahls (firstname.lastname@example.org).
Systematics of parasitic Hymenoptera and DNA-character based identification